Servicebio Electrophoresis Systems

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Electrophoresis Technology

Manufactured by: Servicebio
 
Electrophoresis technology is widely applied across fields such as biological research, analytical and clinical chemistry, toxicology, pharmacology, immunology, and food chemistry. Electrophoresis refers to the migration of charged particles toward electrodes of opposite charge under a direct current electric field. This method enables the separation and analysis of small molecules and is frequently employed in modern medicine to study proteins, nucleic acids, enzymes, and even viral particles.

Beyond protein separation and quantification, electrophoresis has numerous emerging applications in immunology. For example, it is used for the electrophoretic determination of hepatitis B antigen (HBAg) in diagnosing hepatitis B and alpha-fetoprotein (AFP) in detecting primary liver cancer.

This versatile technology continues to play a critical role in research and clinical diagnostics, offering precise, reliable, and reproducible results for a wide range of molecular analyses.

Parameters

Cat. No. BE-BVE-4 BE-BVE-2 BE-BSE-4 BE-SVE-2
Product Name Vertical Electrophoresis System Vertical Electrophoresis System Vertical Electrophoresis System Vertical Electrophoresis System
Number of Gels 1-4 1-2 1-4 1-2
External Dimensions 140x180x185mm 140x180x185mm 153x183x159mm 185x108x125mm
Comb Specifications 1.0mm: 11 teeth / 15 teeth
1.5mm: 11 teeth / 15 teeth
0.75mm: 11 teeth / 15 teeth		 1.0mm: 11 teeth / 15 teeth
1.5mm: 11 teeth / 15 teeth
0.75mm: 11 teeth / 15 teeth		 1.0mm: 11 teeth / 15 teeth
1.5mm: 11 teeth / 15 teeth
0.75mm: 11 teeth / 15 teeth		 1.0mm: 11 teeth / 15 teeth
1.5mm: 11 teeth / 15 teeth
0.75mm: 11 teeth / 15 teeth
Maximum Buffer Capacity 1.5 L 1.5 L 1.6 L 500 mL
Gel Preparation Method Split-type Split-type Integrated Integrated


Cat. No.：BE-BVE-2

Integrated Gel Preparation
Supports up to 2 gels for electrophoresis simultaneously
Clamp-type Snap-fit Design
Multiple anti-reverse designs
Marked liquid level lines on the tank surface
 
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Product Features
 
  • Clamp-Type Bayonet Mount Design – Ensures easy and secure installation of precast gel plates.
  • High-Precision Glass Alignment – Provides uniform surface contact and even force distribution.
  • Graduated Level Markers – Prevents buffer overfill and reduces the risk of electrical shorts.
  • Multi-Polarity Protection – Protects against reverse electrode installation.
  • Compatibility – Fully compatible with BE-BVT-4 and BE-BVT-2 transfer electrophoresis systems.

Cat. No.：BE-BVE-4

Split-type gel preparation
Supports up to 4 gels for electrophoresis simultaneously
Clamp-type buckle design
Multiple anti-reverse designs
Marked liquid level lines on the tank surface
 
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Product Features
 
  • Independent Gel Preparation Mode – Ensures stable and reliable performance while maintaining full compatibility with prefabricated gels of matching models and specifications.
  • Compact, Space-Saving Design – The uniquely designed gel preparation frame allows flexible combinations and efficient use of laboratory space.
  • Versatile Adaptability – Compatible with various glass plate and sample comb thicknesses (1.5 mm, 1.0 mm, 0.75 mm) to meet diverse application needs.
  • High Throughput – Supports simultaneous electrophoresis of two or four gels, improving experimental efficiency.
  • System Compatibility – Designed for seamless integration with BE-BVT-4 and BE-BVT-2 transfer electrophoresis systems.

Cat. No.：BE-SVE-2

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Product Features
 
  • In-Situ Gel Casting Mode – Enables gel casting and electrophoresis without disassembling or reassembling the glass plates. Fully compatible with precast gels of the same model and specifications.
  • Secure Base Locking System – Specially designed base uses non-spring buckles to prevent gel leakage caused by insufficient spring pressure or spring fatigue.
  • Leak-Proof Card Plate Design – The uniquely engineered card plate, combined with the integrated gland, seals the glass plates by compressing the sealing strip, effectively preventing side leakage.
  • Versatile Compatibility – Accommodates glass plates and combs of various thicknesses (1.5 mm, 1.0 mm, 0.75 mm) to support different sample volumes.
  • Low Buffer Requirement – Requires as little as 300 mL of electrophoresis buffer to complete an experiment, reducing reagent consumption.
  • System Integration – Compatible with the BE-SVT-2 transfer electrophoresis tank for streamlined downstream applications.

Product Specifications

BE-SVE-2 BE-SVT-2
Dimensions (L*W*H) mm 185x108x125 185x108x125
Gel size/transfer size (mm) Gel size 83x73 Transfer size 70x90
Maximum volume of buffer (ml) 500 500


4. Operation Steps
This electrophoresis tank is designed to perform gel preparation and electrophoresis for two gels simultaneously.
Before beginning gel preparation, ensure that the concave glass, flat glass, main frame, gel casting assembly, and all related components are thoroughly cleaned and completely dry. Verify that all glass edges are intact and free from chips or damage to ensure optimal sealing and performance.
4.1 Preparation
a. Begin by sliding both side card plates downward to the bottom position and fully opening the cover on both sides.
b. Insert the concave glass and the flat glass diagonally from the top, guiding them carefully into place until they rest at the bottom.
c. Ensure that the upper edges of the glass plates are properly aligned and securely positioned within the card slots on both sides.
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d. Lift the glands on both sides. Using both hands, pinch the left side of the gland and pull the left card plate upward until it locks into place with a “click.” Then, repeat the same operation on the right side—pinch the gland and pull the right card plate upward until a “click” sound confirms proper engagement.
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Notice:
Before starting the experiment, ensure that:
  • The concave and flat glass plates are properly aligned at both the top and bottom.
  • The bottom edges of the glass plates are fully seated in position.
  • The pressure plates and locking buckles at both the upper and lower ends are securely fastened.
Proper alignment and secure installation are essential to prevent leakage and ensure reliable electrophoresis performance.

e. After confirming that the electrophoresis glass plates are properly aligned and securely positioned, loosen the knobs on both sides of the gel base (as shown on the right side of the knob in the illustration below).

Next, place the electrophoresis support in the center of the gel base and secure it in position. Press down gently on the main support with both hands, then tighten the knobs on both sides until they reach their limit (as shown on the left side of the knob).

Note:
The main electrophoresis stand can be installed and used on either side of the base.
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4.2 Gel Preparation
Slowly inject the prepared gel solution into the space between the two glass plates, filling approximately two-thirds to three-quarters of the gel chamber (depending on experimental requirements). Then, gently overlay the surface with double-distilled water or anhydrous ethanol to ensure complete sealing and even surface leveling.

When adding the overlay, proceed carefully to avoid introducing air bubbles. Allow the gel to polymerize for approximately 20 minutes to 1 hour. Once polymerization is complete, pour off the water or ethanol and gently remove any remaining liquid with absorbent paper.

Next, fill the gel chamber (up to the top edge of the glass plates) with the stacking gel solution and insert the sample loading comb. Allow the gel to polymerize completely for 30–45 minutes before proceeding.
 
4.3 Sample Loading
Loosen the knobs on both sides of the gel base (as shown on the right side of the knob in the previous figure). Remove the electrophoresis support assembly and place it carefully into the electrophoresis tank.

The base of the tank includes buckles on both sides, with one side featuring an anti-reversal column to ensure correct orientation. Align the buckles and anti-reversal column simultaneously, then insert the assembly at an approximately 20° angle.

Ensure that the red and black markings on the assembly correspond correctly to the red and black electrode terminals to maintain proper polarity during operation.

Carefully pour the electrophoresis buffer into the main chamber of the electrophoresis stand (between the two sets of glass plates) until the liquid level reaches the upper edge of the concave glass.

Gently remove the sample loading comb from the gel. Inspect each sample well to ensure that no residual gel or air bubbles are present. If residual gel is detected, use a blowpipe to clear it, and carefully remove any trapped air bubbles.

Load the prepared samples into the wells according to experimental requirements to complete the sample loading step.
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4.4 Electrophoresis
Place the top cover onto the electrophoresis tank, ensuring correct alignment of the positive (red) and negative (black) electrodes. Connect the electrophoresis cables to the power supply, matching the corresponding colors.
Set the appropriate voltage and current parameters based on experimental conditions, then start the electrophoresis run.

(Note: Adjust electrophoresis parameters according to specific gel type and experimental protocol.)

5. Product configuration list

Part Numbers Name Quantity
1 Electrophoresis scaffold body 1 Set
2 Gel base 1 Set
3 Electrophoresis box 1 Set
4 1.0 mm electrophoretic glass 2 Sets
5 1.5 mm electrophoretic glass 2 Sets
6 1.0 mm 11 tooth sample adding comb 2 Pcs
7 1.5 mm 11-tooth sampling comb 2 Pcs
8 Glass replacement plate 1 Pcs
9 Gel cutting board 3 Pcs
10 Prefabricated gel seal 2 Pcs


Precautions
1. The main components of this series of products are fragile products. During packaging, transportation and use, be careful not to bump or drop the product, which may cause damage to the product and cause the product to fail to work normally.
2. Metal parts such as platinum wire and electrode column in this product should be paid attention to whether there are oxidation, breakage, loosening and other problems during use, cleaning and maintenance. Please replace and maintain them in time. The platinum wire is easily broken, so be careful when handling it.
3. After daily use, please wash it with deionized water in time, and place it in a dust-free place to dry for use.

Precautions

1. Fragile Components
The primary components of this product series are made of fragile materials. During packaging, transportation, and use, handle the equipment with care. Avoid impact or dropping, as this may cause damage and prevent the product from functioning properly.

2. Metal Parts Maintenance
Regularly inspect metal components—such as the platinum wire and electrode columns—for signs of oxidation, breakage, or loosening during use, cleaning, and maintenance. Replace or service any damaged parts promptly. The platinum wire is particularly delicate; handle it with caution to prevent breakage.

3. Cleaning and Storage
After each use, rinse all components thoroughly with deionized water and allow them to dry in a clean, dust-free environment before storage or reuse.

5. Product configuration list

Catalog Number Product Description Specifications
BE-G6051-1.0 Concave Glass Plate, 1.0 mm (for Liuyi, with reference line) 1.0 mm
BE-G6051-1.0T Glass Plate Set, 1.0 mm (for Liuyi, with reference line) 1.0 mm
BE-G6051-1.5 Concave Glass Plate, 1.5 mm (for Liuyi, with reference line) 1.5 mm
BE-G6051-1.5T Glass Plate Set, 1.5 mm (for Liuyi, with reference line) 1.5 mm
BE-G6051-P Flat Glass Plate Flat Glass Plate
BE-G6052-0.75 Concave Glass Plate, 0.75 mm (for Servicebio & Bio-Rad, with reference line) 0.75 mm
BE-G6052-0.75T Glass Plate Set, 0.75 mm (for Servicebio and Bio-Rad, with reference line) 0.75 mm
BE-G6052-1.0 Concave Glass Plate, 1.0 mm (for Servicebio and Bio-Rad, with reference line) 1.0 mm
BE-G6052-1.0T Glass Plate Set, 1.0 mm (for Servicebio & Bio-Rad, with reference line) 1.0 mm
BE-G6052-1.5 Concave Glass Plate (for Servicebio & Bio-Rad, with reference line) 1.5 mm
BE-G6052-1.5T Glass Plate Set, 1.5 mm (for Servicebio & Bio-Rad, with reference line) 1.5 mm